dsdA [2019-01-28 15:57:51]

D-serine deaminase

locus: BSU23770

pI: 5.55

mw: 49.50 kDa

protein length: 448 aa

gene length: 1344 bp

function: unknown

product: D-serine deaminase

essential: no

ec: 4.3.1.18

synonyms: yqjR

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,469,580 → 2,470,926

The protein

Catalyzed reaction/ biological activity

• D-serine = pyruvate + NH₃ (according to Swiss-Prot)

Protein family

• DsdA subfamily (according to Swiss-Prot)

Structure

• [PDB|3SS7] (from E. coli, 58% identity) [pubmed|22197591]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|35B40D812B123766B469B8E5C0FE0EB69F671E4E|yqjP]-[gene|0927C7D45721F47F5ED2646CAAC324A308FD7724|yqjQ]-[gene|4E7390A74A8261E8B732E94859E90FE256AD64EE|dsdA]-[gene|B3362449EF3C9D533090FBD7E7333A3CC5D902CB|coaA]-[gene|441DCE4251C83FCBB01BC58720900365BFE7BB3D|yqjT]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C398 (yqjR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2396 NBRP B. subtilis, Japan]
• BKE23770 (Δ[gene|4E7390A74A8261E8B732E94859E90FE256AD64EE|dsdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAACAATCTCTCCCTT, downstream forward: _UP4_TAAGCAGACAGTGAAAAGGT
• BKK23770 (Δ[gene|4E7390A74A8261E8B732E94859E90FE256AD64EE|dsdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAACAATCTCTCCCTT, downstream forward: _UP4_TAAGCAGACAGTGAAAAGGT

References

• 3098560,22197591